Resources and information
BioAcademy Handbook
A resource for Students, Local Instructors, Labs, and Advisors
Hardware, reagents and software overview
In this doc you can find all the reagents and hardware
Synthetic Biology One video's
Synthetic Biology One http://syntheticbiology1.com/ is a free, open, online course. They don't precisely match the HTGAA curriculum, but the spirit is the same and the overall content is very similar. So if you sign up and follow the courses, you will get a different perspective on the same stuff.
You can also cherry-pick videos from the YouTube channel, on selected topics. this list of videos might be interesting, organized by week of HTGAA.
HTGAA Week 1
- The Field of Synthetic Biology
- All About Standard Biological Parts
- The Parts of a Plasmid
- The Features of a Coding Sequence
- A Modular View of Gene Expression
- Bacterial Growth Requirements
- Common Units of Concentration in the Lab
- Calculating Dilutions in the Lab
- Finding Interesting DNA Sequences in GenBank
- A Digital Plasmid in Sequence Editor Software
- Decoding Functional DNA with BLAST
HTGAA Week 2
- The Theory of PCR
- How to Set Up a PCR
- How to Screen Bacterial Colonies with PCR
- Troubleshooting a Bad PCR
- How to Make an Agarose Gel
- How to Load an Agarose Gel
- How to Perform a PCR Purification
- How to Quantify DNA with a Spectrophotometer
HTGAA Week 3
- Cut and Paste DNA
- Introduction to Restriction Enzyme Cloning
- All About Enzymatic Digests of DNA
- How to Identify a Plasmid by Analytical Digest
- Directional Cloning and BioBricks
- How to Make Chemically Competent E. coli
- How to Transform E. coli by Heat Shock
- How to Cut DNA from an Agarose Gel
- How to Ligate and Transform DNA Fragments
- How to Harvest Plasmid DNA with Minipreps
HTGAA Week 4
- How to Make LB, a Standard Bacterial Growth Medium
- How to Streak for Single Colonies
- Let's Model Exponential Growth
- Let's Model Logistic Growth
- Measuring Bacterial Growth by Optical Density
- Logistic Collector: The Growth Curve Game
- How to Store Microbes as Glycerol Stocks
- How to Count Bacteria in Yogurt
HTGAA Week 5
- Choosing the GFP Variant That's Right for You
- How to Measure Fluorescence in Bacterial Cultures
- Working with Arbitrary Units
- Model mRNA Transcription Rates
- Let's Model Protein Translation Rates
- Michaelis Menten Kinetics
- Steady State and the Equilibrium Constant
- Substrates and Products in Chemical Reactions
Finally, if you are in China, you can get us on our Bilibili channel Some of those videos also have subtitles in Cantonese or Mandarin.