How do you tailor the metabolism of a microbe to make a product? People have been tweaking microbial metabolism since the advent of fermented foods like beer, bread, and cheese. Over the last century, new tools have been transforming this process into an engineering discipline. In this class, we will explore the latest tools and approaches to designing microbial metabolism.
How does engineering in biology differ from engineering in other fields? What design principles can be applied to microbes, which are products of evolution? How do we find good biological parts? How do we know if an engineered microbe is performing as expected? We will review Design, Build, and Test strategies appropriate for engineering the metabolism of microbes.
PDFs available if requested
Bonus Background reading
Software (not required for assignment)
Design a biosynthetic route to produce a small molecule in an organism. See the class slides for tools and examples. Choose a target compound and answer the following questions:
1. What chassis and pathway will you use? 2. How will you measure product formation? 3. What chassis enzymes would you modify?