Table of Contents

Bio production

23 September videos (review4 on vimeo, lesson5 on vimeo) - Patrick Boyle


How do you engineer a microbe to produce an interesting small molecule? What design principles can be applied to un-designed (i.e. evolved) microbes? How do we find good biological parts? How do we know if an engineered microbe is performing as expected? We will review Design, Build, and Test strategies appropriate for engineering the metabolism of bacteria and yeast.

Lecture slides

patrick_htgaa_092315.pdf

Background reading


PDFs available if requested

General review of synthetic biology tools and methods for metabolic engineering

Commentary on lessons learned during the development of synthetic chemistry, and how that may influence the development of synthetic biology

Bonus Background reading

Recent publication on the production of opiods in yeast

Publication on the use of DNA synthesis and bioinformatics to discover novel enzymes

Software (not required for assignment)

Open source software for modeling metabolic networks