Write a Python script that outputs the sequences with dumbbell insertion 'TCCTCTTTTGAGGAACAAGTTTTCTTGT' exactly in the middle of each strand in 96-well format according to
Ideally at least two teams achieve this goal, so you can cross-validate your results against each other to gain confidence that the design is correct
Extra-extra credit homework assignment
Pool resources to order collectively from IDT the four 96-well plates needed to render 1-bit patterns
~$1500–$2000 for this set of strands on the 10 nmol scale (request 6 nmol total: 60 µL at 100 µM in water)
Assuming each pattern will consume 10 pmol per strand, this will be enough material to fold 600 patterns
If anybody makes it this far in the assignment, I (i.e. William Shih) will proofread your order and assist with formatting your order to IDT, give you more detailed protocols for folding and gel analysis (although you can find this all in the primary literature assigned as reading for this class)
Pipet strands (10 pmol each) to generate all patterns submitted by teams in the class
Fold patterns in a thermocycler in a volume of 100 µL per pattern
Check to see that the structures fold correctly by agarose-gel analysis of 15 µL aliquot
Find a partner (commercial or otherwise) willing to do AFM imaging of your samples
Submit your samples to that partner (only 1 µL required for imaging)