====== Bio production ======

**23 September [[http://connectmedia.waag.org/AcadeMany/bio-2015/09/23/|videos]] ([[https://vimeo.com/140202290|review4 on vimeo]], [[https://vimeo.com/140203045|lesson5 on vimeo]]) - Patrick Boyle**

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How do you engineer a microbe to produce an interesting small molecule? What design principles can be applied to un-designed (i.e. evolved) microbes? How do we find good biological parts? How do we know if an engineered microbe is performing as expected? We will review Design, Build, and Test strategies appropriate for engineering the metabolism of bacteria and yeast.
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===== Lecture slides =====
{{:patrick_htgaa_092315.pdf|}}

===== Background reading =====
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PDFs available if requested
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[[http://www.ncbi.nlm.nih.gov/pubmed/22037345|General review of synthetic biology tools and methods for metabolic engineering]]

[[http://www.ncbi.nlm.nih.gov/pubmed/17710092|Commentary on lessons learned during the development of synthetic chemistry, and how that may influence the development of synthetic biology]]

==== Bonus Background reading ====
[[https://www.sciencemag.org/content/349/6252/1095.abstract|Recent publication on the production of opiods in yeast]]

[[http://pubs.acs.org/doi/abs/10.1021/ja809461u|Publication on the use of DNA synthesis and bioinformatics to discover novel enzymes]]

==== Software (not required for assignment) ====
[[http://opencobra.sourceforge.net/openCOBRA/Welcome.html|Open source software for modeling metabolic networks]]