====== Computational protein design, biosensors and the protein-folding game ====== **November 18 -- [[http://connectmedia.waag.org/AcadeMany/bio-2015/11/18/|videos]] ([[https://vimeo.com/146139576|review12 on vimeo]], [[https://vimeo.com/146156498|lesson13 on vimeo]]) -- Srivatsan Raman (University of Wisconsin-Madison)** Protein science is entering an exciting new phase enabled by major advances in computational and experimental methods. Computational protein modeling has achieved unprecedented accuracy in predicting protein structures at atomic-level accuracy, and designing proteins as biocatalysts, with new binding partners (protein-protein, protein-DNA, protein-ligand) and self-assembling materials. On the experimental side, with next generation technologies to synthesize and sequence DNA, we can evaluate millions of protein designs in a single tube, and quantify function by high-throughput sequencing. By combining computational modeling and high-throughput experiments, we can design, build and test a large number of candidates, and select the best performing design. In previous lectures, you have seen how methods like CRISPR-Cas9, MAGE and large DNA assembly allow us to edit genomes, mutate enzymes or make large refactored biosynthetic operons. Technologies to read DNA (next gen sequencing) and write DNA (CRISPR, MAGE etc) is far ahead of our ability evaluate phenotype or function. For instance, using MAGE we can make a billion variants of a biosynthetic pathway in a single day. But the question is, how do we know which one of these cells is the highest producer of your target molecule? Enter biosensors. Biosensors are genetically encoded sensors that are specific to the target molecule and report concentration in each cell, allowing us to select the highest producer by high-throughput sorting or genetic selection. In this class we will cover three topics: 1. Computational methods for modeling protein structures and interactions. We will cover tools and apps in the Rosetta protein modeling suite. http://www.ncbi.nlm.nih.gov/pubmed/18410248 2. Application of biosensors for high-throughput metabolic engineering. http://www.ncbi.nlm.nih.gov/pubmed/20367033 We will also discuss how to design a brand new biosensor for a molecule. 3. FoldIt, protein folding game. Hands on demo of the FoldIt video game. https://www.youtube.com/watch?v=lGYJyur4FUA **HOME WORK** The objective of the homework is to run the Rosetta protein structure prediction simulations and analyze the results.The idea is to get an understanding of how computational protein modeling works, looking at protein structures using a viewer (PyMol or Chimera or Rasmol) and making sense of the squiggles and wiggles. You'll also hopefully appreciate the computing power needed for biomolecular simulations. You'll pick one of the five test cases in the homework folder and run structure prediction calculations. The native structure of all the five test cases has already been solved. So, you can compare your output against the correct answer. __Description of the folder contents__ The homework/structure_prediction has seven subfolders: 1S12, 1TTZ, 1WHZ, 2HFQ, 2HJJ, database and executable The executable folder contains two executables one each for Mac and Linux. There is no executable for Windows machine. You have to run the calculations on a Mac or Linux terminal. The database folder has a ton of precomputed database files that Rosetta uses for the simulation. The remaining five folders are the test cases. You'll pick one for your homework (feel free to pick more than one). The name of the folder is the PDB (www.rcsb.org) code of the native structure. __Contents of the test case folders (1S12,1TTZ, 1WHZ, 2HFQ, 2HJJ):__ In each folder you'll find six files. 'XXXX' corresponds to any one of the above PDB codes. XXXX.200.3mers and XXXX.200.9mers: these are database of short fragments that Rosetta uses to assemble 3D structure customized for this protein. XXXX.fasta: this is the protein sequence. XXXX.pdb: this is the experimentally solved native structure. XXXX.psipred.ss2: this contains the secondary structure propensity at every position. abrelax_flags: instructions for the Rosetta code and paths to database. The only file you may have to modify is abrelax_flags. You won't have to touch any of the other files. Running the calculations Pick one test case. You can look at the native structure topology and size, and may be even function and decide on which one to pick. On a linux or mac terminal, navigate your way to the homework folder. Go to the specific folder of your test case. Open abrelax_flags in your favorite editor. Check to see if the following line is as shown. -database ../database If it says anything else besides ../database change it to ../database The line -nstruct 100 tells the code to generate a 100 models. The more models you generate, the accurate your result is likely to be. But running more models requires more computing time. So you'll have balance that. The first time see how many hours it takes to generate a hundred models. If you have more free computer time, change the nstruct 100 to a larger number and rerun the calculations. To the run program, type the following command inside your specific test case folder. If you type this anywhere else the program will not run because its looking for specific files in that folder. ../executable/AbinitioRelax.static.macosclangrelease @abrelax_flags That should start the program, and it will keep running till it generates a 100 models. If you turn off your computer or close the terminal screen, the job will end. If you are bit linux savvy, you can use the 'nohup' command and free the terminal. After about 10-15 mins, you should additional files appear in that directory. score.fsc S_000001.pdb, S_000002.pdb etc The S_ files are the models. The score.fsc file contains energy (in the 'score' column) and distance to native (in the 'rms' column). __Deliverables for homework assignment__ 1. Plot the score (or energy) vs rms plot. Rms stands for root mean square deviation. These are two columns in the score.fsc file. Compare that with the energy vs rms plots I showed in my slides. 2. Pick the lowest energy model and structurally compare it to the native. How close is it to the native? If its different, what parts did the computer program get wrong? You'll have to compare the structures using a Viewer like pymol or chimera or rasmol. 3. Pick the lowest rms model and structurally compare it to the native. How close is it to the native? If its different, how is it different? Remember that in a blind case, we will not have the benefit of an rms column.